Multiple Grammy winners Macklemore & Ryan Lewis performed their hit "Same Love" at the awards ceremony as 34 couples in the audience, including Lewis' sister and her boyfriend tied the knot in a ceremony presided over by Queen Latifah.

Latifah shouted, "I now pronounce you married!" as Madonna joined the rap duo, accompanied by Mary Lambert, onstage. The couples were described by The New York Times as "gay, straight, old, young, of many races and many colors."

"A night that is already tremendous for me, for the music," said Lewis before the ceremony, "but to have my sister get married and my family there watching it—that makes it a whole other level of amazing."

Madonna then sang "Open Your Heart" as the just-married couples embraced and smiled for the cameras. Watch the performance below.

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